Characterization of cDNAs Encoding Small and Large Subunits of ADP-Glucose Pyrophosphorylases from Watermelon (Citrullus vulgaris S.).
نویسندگان
چکیده
منابع مشابه
Two negative cis-regulatory regions involved in fruit-specific promoter activity from watermelon (Citrullus vulgaris S.)
A 1.8 kb 5'-flanking region of the large subunit of ADP-glucose pyrophosphorylase, isolated from watermelon (Citrullus vulgaris S.), has fruit-specific promoter activity in transgenic tomato plants. Two negative regulatory regions, from -986 to -959 and from -472 to -424, were identified in this promoter region by fine deletion analyses. Removal of both regions led to constitutive expression in...
متن کاملCharacterization of cDNAs encoding small GTP-binding proteins from maize.
To date, more than 100 genes encoding small GTP-binding proteins (21-25 kD) have been identified from various eukaryotic organisms, including mammals, insects, yeasts, and higher plants (Hall, 1990; Bourne et al., 1991; Terryn et al., 1993). These proteins, which contain highly homologous regions that have been conserved during evolution, are conveniently classified into three major groups; ras...
متن کاملPurification and Properties of Glyoxysomal Cuprozinc Superoxide Dismutase from Watermelon Cotyledons (Citrullus vulgaris Schrad).
A glyoxysomal copper,zinc-containing superoxide dismutase (EC 1.15.1.1) was purified to homogeneity, for the first time, from watermelon cotyledons (Citrullus vulgaris Schrad.). The stepwise purification procedure consisted of acetone precipitation, batch anion-exchange chromatography, anion-exchange Fast Protein Liquid Chromatography and gel-filtration column chromatography. Pure copper,zinc-s...
متن کاملInvestigation of the Interaction between the Large and Small Subunits of Potato ADP-Glucose Pyrophosphorylase
ADP-glucose pyrophosphorylase (AGPase), a key allosteric enzyme involved in higher plant starch biosynthesis, is composed of pairs of large (LS) and small subunits (SS). Current evidence indicates that the two subunit types play distinct roles in enzyme function. Recently the heterotetrameric structure of potato AGPase has been modeled. In the current study, we have applied the molecular mechan...
متن کاملCloning and characterization of cDNAs encoding the integrin α2 and α3 subunits from Xenopus laevis
Integrins containing the a2 and a3 subunits associate with the b1 subunit to form distinct receptors with partially overlapping adhesive specificities. We report the cloning and sequence of cDNAs that encode the Xenopus orthologues of integrins a2 and a3 and the expression of these subunits during embryogenesis. Integrin a2 and a3 mRNAs are first expressed in the dorsal mesoderm and developing ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 1998
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.62.550